Trifluoroacetic Acid
Trifluoroacetic acid is a corrosive organofluorine compound that is structurally analogous to, but stronger than, acetic acid. Available in various quantities and reagent grades, it is used in NMR spectroscopy, mass spectrometry, organic synthesis, etc.
Almost 100,000-fold more acidic than acetic acid, TFA is widely used in organic chemistry. TFA is used as a reagent in organic synthesis because of its properties: volatility, organic solvent solubility, and acidic strength. It is less oxidizing than sulfuric acid and is more readily available in its anhydrous form than some other acids . Other features include:
- Used in acid-catalyzed reactions, especially peptide synthesis (to cleave esters)
- Dissolves protein when mixed with liquid SO2
- Removes t-butyl derived side-chain protecting groups in Fmoc peptide synthesis
- Can remove the t-butoxycarbonyl protecting group in organic synthesis
- At low concentrations, acts as an ion-pairing agent for peptides and small proteins in organic compound liquid chromatography
- For acid-stable materials, can be a solvent for NMR spectroscopy
- Acts as a calibrant in mass spectrometry
- Used to produce trifluoroacetate salts
- An ingredient in adhesives, sealants, paints, and coatings
When TFA combines with bases and metals, especially light metals, a strong exothermic reaction occurs. When mixed with lithium aluminum hydride (LAH), the reaction is explosive.
Although nonflammable, TFA is corrosive to skin, eyes, and mucous membranes and requires careful use and handling. It is harmful when inhaled, causes severe skin burns and eye damage, and is toxic to aquatic organisms at even low concentrations.
TFA is also a product of the metabolic breakdown of the anesthetic agent halothane. It is the suspected cause of halothane-induced hepatitis.
Medchemexpress LLC WAAG-3R TFA | 99.8% | 1644.74 | 1 MG
WAAG-3R TFA is a biologically active peptide. Aggrecanases, which are part of the ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases, cleave aggrecan, a main structural component of cartilage. Aggrecanase-1 (ADAMTS-4) is a significant aggrecanase in human osteoarthritic cartilage. This FRET peptide was utilized in an ADAMTS-4 (Aggrecanase-1) and ADAMTS-5 (Aggrecanase-2) assay with Ex/Em = 340/420 nm.
- Biologically active peptide.
- Utilized in an ADAMTS-4 (Aggrecanase-1) and ADAMTS-5 (Aggrecanase-2) assay.
- FRET peptide with Ex/Em = 340/420 nm.
Medchemexpress LLC Survodutide TFA | 99.4% | 4231.62 (free base) | 25 MG
Survodutide TFA is a potent and selective dual agonist targeting the glucagon receptor (GCGR) and GLP-1 receptor (GLP-1R), with EC50s of 0.52 nM and 0.33 nM respectively in CHO-K1 cells. This 29-amino-acid acylated peptide, featuring a C18 fatty acid, demonstrates significant anti-obesity efficacy by enhancing energy expenditure and reducing food intake.
- Potent, selective GCGR/GLP-1R dual agonist
- EC50s of 0.52 nM (GCGR) and 0.33 nM (GLP-1R)
- 29-amino-acid acylated peptide
- Contains a C18 fatty acid
- Demonstrates robust anti-obesity efficacy
- Increases energy expenditure and decreases food intake
- Enhanced proteolytic stability
- Extended terminal half-life
Medchemexpress LLC L-lysine, L-lysyl-L-glutaminyl-L-phenylalanyl- | 99.6% | 891.73 | 25 MG
KQFK TFA is a peptide, the trifluoroacetate salt form of KQFK, and serves as an inactive control for KRFK, a peptide derived from TSP-1. This product is for research use only and has not been fully validated for medical applications.
- Molecular formula: C32H46F9N7O12
- Molecular weight: 891.73
- Purity (LCMS): 99.55%
- Appearance: White to off-white solid
- Storage: Powder at -80°C for 2 years or -20°C for 1 year; in solvent at -80°C for 6 months or -20°C for 1 month (sealed, away from moisture)
Medchemexpress LLC Wl47 (TFA) | 99.7% | 1942.32 | 5 MG
WL47 TFA is a high-affinity cavolin-1 (CAV1) ligand that potently disrupts CAV1 oligomers. It shows selectivity for CAV1 over BSA, casein, and HEWL, and is used for studying caveolin-1 function. It is also 80% smaller in length than the original T20 parent sequence.
- High-affinity cavolin-1 (CAV1) ligand (Kd=23 nM)
- Potently disrupts CAV1 oligomers
- Exhibits selectivity for CAV1 over BSA, casein, and HEWL
- Used for studying caveolin-1 function
- 80% smaller in length than original T20 parent sequence
Medchemexpress LLC dTAGV-1 TFA | 2624313-15-9 | 99.9% | C70H91F3N6O16S | 1 MG
dTAGV-1 TFA is a potent and selective PROTAC degrader targeting FKBP12F36V-tagged proteins. It is capable of inducing degradation of FKBP12F36V-Nluc both in vitro in 293FT cells and in vivo in animal models.
- Potent and selective degrader of FKBP12F36V-tagged proteins.
- Induces degradation of FKBP12F36V-Nluc in vivo.
- Effective in 293FT cells.
- Reduces bioluminescent signal in animal models.
Medchemexpress LLC Amg 7905 (tfa) | 00-00-0 | 99.1% | 597.58 | C27H25F6N5O2S | 5 MG
AMG 7905 TFA is the trifluoroacetate salt of AMG 7905, a potent transient receptor potential vanilloid 1 (TRPV1) antagonist used in research. It potentiates proton-enhanced TRPV1 activation while blocking capsaicin-induced channel responses, and has been used to study thermoregulation and TRP channel physiology. The compound is supplied as a high-purity research reagent for in vitro and in vivo studies.
- Potent TRPV1 antagonist activity.
- Modulates proton-enhanced channel activation and blocks capsaicin responses.
- High purity (≈99.1%).
- Provided as the trifluoroacetate (TFA) salt to aid handling and solubility.
- Available in small research pack sizes suitable for pilot studies.
- Intended for laboratory research use only.
Medchemexpress LLC Lys-bradykinin trifluoroacetate | 99.9% | 1188.4 g/mol | C56H85N17O12 · x C2HF3O2 | 5 MG
Lys-bradykinin trifluoroacetate is the trifluoroacetic acid (TFA) salt form of the peptide Lys-Bradykinin (kallidin 380-389). It is supplied as a purified powder for research applications where it serves as a ligand for kallidin and bradykinin receptors. Reported purity is 99.85% and the free-base molecular weight is 1188.38 g/mol.
- TFA salt of Lys-Bradykinin peptide.
- High purity (99.85%).
- Provided as a solid powder suitable for dissolution in common solvents.
- Recommended storage: sealed, protected from moisture and light; powder stable at -80°C for up to 2 years.
- Intended for research use as a ligand in receptor binding or signaling studies.
Medchemexpress LLC Valylleucine TFA (Val-Leu TFA) | 27530-02-5 | 99.3% | 344.33 | 5 MG
Valylleucine TFA is a polypeptide identified through peptide screening. Peptide screening serves as a research tool, primarily utilizing immunoassay to pool active peptides. This process is useful for studying protein interaction, functional analysis, and epitope screening, particularly in the development of agents.
- Identified through peptide screening
- Research tool primarily using immunoassay to pool active peptides
- Useful for studying protein interaction, functional analysis, and epitope screening
- Solutions should be aliquoted and stored to prevent inactivation from repeated freeze-thaw cycles
- If water is used as stock solution, dilute to working solution, then filter and sterilize with a 0.22 μm filter before use
Sigma Aldrich Fine Chemicals Biosciences Trifluoroacetic acid 99 fo500G
TRIFLUOROACETIC Trifluoroacetic acid 99 fo500G
Medchemexpress LLC Maurocalcine (TFA) | 95.1% | 3858.55 (free base) | 100 UG
Maurocalcine TFA is an agonist of ryanodine receptor (RyR) channel types 1, 2 and 3 with cellular permeability. It induces [3H]ryanodine binding on RyR1 with an EC50 value of 2558 nM. This product exhibits an apparent affinity of 14 nM for RyR2 and can be applied to in vivo cell tracking or other cell imaging techniques.
- Agonist of ryanodine receptor (RyR) channel types 1, 2 and 3
- Exhibits cellular permeability
- Induces [3H]ryanodine binding on RyR1 with an EC50 value of 2558 nM
- Exhibits an apparent affinity of 14 nM for RyR2
- Can be applied to in vivo cell tracking or other cell imaging techniques
Medchemexpress LLC Alvespimycin trifluoroacetate | 00-00-0 | MFCD08457919 | 99.0% | 730.77 g/mol | C34H49F3N4O10 | 1 MG
Alvespimycin TFA is the trifluoroacetate salt of 17-DMAG, a small-molecule Hsp90 inhibitor used in research to probe Hsp90 biology, induce client protein degradation, and trigger stress responses in cancer cell models.
- Potent Hsp90 inhibition with low-nanomolar cellular activity.
- Promotes degradation of Hsp90 client proteins, including Her2.
- Induces Hsp70 expression and apoptosis in sensitive cancer cells.
- High purity solid suitable for biochemical and cellular assays.
- Available in small research quantities for dose-response studies.
- Stable when stored sealed at recommended temperatures.
Medchemexpress LLC KRpep-2d TFA | 2098181-84-9 | 95.09% | 2675.03 | 25 MG
KRpep-2d TFA is a potent K-Ras inhibitor that inhibits the proliferation of K-Ras-driven cancer cells. It possesses a cyclic structure, with specific amino acid residues (Leu77, Ile9, and Asp12) being critical for its K-Ras inhibitory activity. This peptide can be used for cancer research.
- Potent K-Ras inhibitor
- Inhibits proliferation of K-Ras-driven cancer cells
- Cyclic structure important for inhibitory activity
- Demonstrates inhibitory activity in A427 cells
- Targets Ras
- Involved in GPCR/G Protein and MAPK/ERK Pathway